RT-PCR and CP gene based molecular characterization of a cucumber mosaic cucumovirus from Aligarh, U.P., India
نویسندگان
چکیده
منابع مشابه
Biolistic inoculation of gladiolus with cucumber mosaic cucumovirus.
A new method of inoculation of gladiolus with cucumber mosaic virus (CMV) was developed using the Bio-Rad Helios Gene Gun System. This method circumvents the traditional use of aphids to transmit CMV, a virus that is mechanically transmissible to many plant species but only with difficulty to gladiolus. Cartridges containing virus-coated gold microcarriers were prepared and the virus shot into ...
متن کاملCharacterization of Cucumber Mosaic Virus From Mint in Tehran Province
Cucumber mosaic virus (CMV) is one of the causal agents of viral diseases that infected mint (Mentha spp.). CMV is a member of the cucumovirus genus in Bromoviridae family. The virus is naturally transmitted by aphids in a non-persistent manner.During growing season 2007-2008, leaf samples from plants of mint fields with symptoms of viral disease were collected in mint fieldas in Tehran provi...
متن کاملCharacterization of Cucumber Mosaic Virus From Mint in Tehran Province
Cucumber mosaic virus (CMV) is one of the causal agents of viral diseases that infected mint (Mentha spp.). CMV is a member of the cucumovirus genus in Bromoviridae family. The virus is naturally transmitted by aphids in a non-persistent manner.During growing season 2007-2008, leaf samples from plants of mint fields with symptoms of viral disease were collected in mint fieldas in Tehran provi...
متن کاملEfficient infection from cDNA clones of cucumber mosaic cucumovirus RNAs in a new plasmid vector.
Full-length cucumber mosaic cucumovirus (CMV) cDNAs were cloned into a new plasmid vector containing a modified plant virus promoter designed to transcribe the inserted sequence from its first nucleotide. cDNA copies of CMV strain Q (Q-CMV) genomic RNAs 1, 2 and 3 cloned into this vector were infectious when inoculated together, producing symptoms indistinguishable from those caused by wildtype...
متن کاملCharacterization of Coat Protein Gene of Cucumber Mosaic Virus Isolates in Iran
Background: Cucumber mosaic virus (CMV) from the Bromoviridae family, is one of the most widespread plant viruses in the world. Objectives: In the present study tomato fields in Guilan, Isfahan, Khorasan Razavi, Khuzestan and Tehran provinces were surveyed to determine the presence of CMV subgroups during 2011-2012. Materials and Methods: Out of 305 symptomatic leaf samples ...
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ژورنال
عنوان ژورنال: Agricultural Sciences
سال: 2012
ISSN: 2156-8553,2156-8561
DOI: 10.4236/as.2012.38118